The Inositol- 1,4,5=Trisphosphate System Is Involved in Rapid Effects of Aldosterone in Human Mononuclear Leukocytes

There is increasing evidence for rapid steroid action on electrolyte transport in human mononuclear leukocytes (HML). In HML, aldosterone stimulates the Na+/H+ antiporter within a few minutes. Because a variety of hormones and growth factors activate the Na+/H+ antiporter via protein kinase C and inositol phospholipids, a possible involvement of inositol-1,4,5-trisphosphate (IP3) in the rapid effects of aldosterone in HML was investigated. The stimulation of IP3 generation was started by the addition of aldosterone, concanavalin A, or other steroids. A significant increase in IP3 levels by aldosterone (1 nmol/L, P < 0.05) was found after 1 min, similar to that found after concanavalin A (25 micrograms/mL). Aldosterone caused a concentration-dependent elevation of IP3 levels, with an apparent EC50 of approximately 0.1 nmol/L. Fludrocortisone stimulated IP3 generation at similar concentrations, whereas a weaker IP3 stimulation by glucocorticoids (hydrocortisone, dexamethasone) occurred at micromolar concentrations only. Canrenone, a potent inhibitor of classical aldosterone action, was not effective up to a concentration of 100 nmol/L. These findings show kinetic and pharmacological similarities with both the functional data on Na+/H+ antiport stimulation by aldosterone and the studies of 125I-aldosterone binding to plasma membranes of HML. Thus, these data are the first to indicate an involvement of the phosphoinositide pathway in the rapid membrane effects of aldosterone

Sediment features at the grounding zone and beneath Ekström Ice Shelf, East Antarctica, imaged using on-ice vibroseis

The grounding zone, where an ice sheet becomes a floating ice shelf, is known to be a key threshold region for ice flow and stability. A better understanding of ice dynamics and sediment transport across such zones will improve knowledge about contemporary and palaeo ice flow, as well as past ice extent. Here we present a set of seismic reflection profiles crossing the grounding zone and continuing to the shelf edge of Ekström Ice Shelf, East Antarctica. Using an on-ice vibroseis source combined with a snowstreamer we have imaged a range of sub-glacial and sub-shelf sedimentary and geomorphological features; from layered sediment deposits to elongated flow features. The acoustic properties of the features as well as their morphology allow us to draw conclusions as to their material properties and origin. These results will eventually be integrated with numerical models of ice dynamics to quantify past and present interactions between ice and the solid Earth in East Antarctica; leading to a better understanding of future contributions of this region to sea-level rise

Critical porosity of gas enclosure in polar firn independent of climate

In order to interpret the paleoclimatic record stored in the air enclosed in polar ice cores, it is crucial to understand the fundamental lock-in process. Within the porous firn, bubbles are sealed continuously until the respective horizontal layer reaches a critical porosity. Present-day firn air models use a postulated temperature dependence of this value as the only parameter to adjust to the surrounding conditions of individual sites. However, no direct measurements of the firn microstructure could confirm these assumptions. Here we show that the critical porosity is a climate-independent constant by providing an extensive data set of micrometer-resolution 3-D X-ray computer tomographic measurements for ice cores representing different extremes of the temperature and accumulation ranges. We demonstrate why indirect measurements suggest a climatic dependence and substantiate our observations by applying percolation theory as a theoretical framework for bubble trapping. The incorporation of our results significantly influences the dating of trace gas records, changing gas-age–ice-age differences by up to more than 1000 years. This may further help resolve inconsistencies, such as differences between East Antarctic δ15N records (as a proxy for firn height) and model results. We expect our findings to be the basis for improved firn air and densification models, leading to lower dating uncertainties. The reduced coupling of proxies and surrounding conditions may allow for more sophisticated reinterpretations of trace gas records in terms of paleoclimatic changes and will benefit the development of new proxies, such as the air content as a marker of local insolation

On-ice vibroseis and snowstreamer systems for geoscientific research

We present implementations of vibroseis system configurations with a snowstreamer for over-ice long-distance seismic traverses (>100 km). The configurations have been evaluated in Antarctica on ice sheet and ice shelf areas in the period 2010–2014. We discuss results of two different vibroseis sources: Failing Y-1100 on skis with a peak force of 120 kN in the frequency range 10–110 Hz; IVI EnviroVibe with a nominal peak force of 66 kN in the nominal frequency range 10–300 Hz. All measurements used a well-established 60 channel 1.5 km snowstreamer for the recording. Employed forces during sweeps were limited to less than 80% of the peak force. Maximum sweep frequencies, with a typical duration of 10 s, were 100 and 250 Hz for the Failing and EnviroVibe, respectively. Three different concepts for source movement were employed: the Failing vibrator was mounted with wheels on skis and pulled by a Pistenbully snow tractor. The EnviroVibe was operated self-propelled on Mattracks on the Antarctic plateau. This lead to difficulties in soft snow. For later implementations the EnviroVibe with tracks was put on a polyethylene (PE) sled. The sled had a hole in the center to lower the vibrator baseplate directly onto the snow surface. With the latter setup, data production varied between 20 km/day for 6-fold and 40 km/day for single fold for 9 h/day of measurements. The combination of tracks with the PE-sled was especially advantageous on hard and rough surfaces because of the flexibility of each component and the relatively lose mounting. The systems presented here are suitable to obtain data of subglacial and sub-seabed sediment layers and englacial layering in comparable quality as obtained from marine geophysics and land-based explosive surveys. The large offset aperture of the streamer overcomes limitations of radar systems for imaging of steep along-track subglacial topography. With joint international scientific and logistic efforts, large-scale mapping of Antarctica's and Greenland's subglacial geology, ice-shelf cavity geometries and sea-bed strata, as well as englacial structures can be achieved

Pre-site Study for Deep Geological Drilling below Ekström Ice Shelf, Sub-EIS-Obs

During the last seasons and ongoing, pre-site seismic surveys have taken place in the Ekströmisen region of Dronning Maud Land, with the primary of building a stratigraphic age framework of the under-ice-shelf sediments. These sediments are overlying the Explora Wedge, a syn- or post-rift volcanic deposit. Expected ages range from Late Mesozoic to Quaternary. From new vibroseismic profiles we will select sites for short core seafloor sampling through Hot Water Drill (HWD) holes of the oldest and of the youngest sediment sequences to confine their age time span. There is further potential for drilling deeper sediment cores with the support of international partner. Deep drilling should recover the sediments overlying the Explora Escarpment, in order to discover the nature of the Explora Wedge. We expect the overlying sediment sequences to reveal the history of polar amplification and climate changes in this part of Antarctica, the build-up of the East Antarctic Ice Sheet during past warmer climates, and its Cenozoic and future variability. Having HWD holes through the shelf ice and sampling the sea floor will provide the unique opportunity for further piggy back experiments consisting of multi-disciplinary nature. Experiments and measuring setup for oceanography, sea and ice shelf physics, geophysics, geology, hydrography, biogeochemistry could be planned to characterize the ocean-ice-sediment interactions, processes and ecosystem observations

Quantifying the effect of ocean bed properties on ice sheet geometry over 40 000 years with a full-Stokes model

Simulations of ice sheet evolution over glacial cycles require integration of observational constraints using ensemble studies with fast ice sheet models. These include physical parameterisations with uncertainties, for example, relating to grounding-line migration. More complete ice dynamic models are slow and have thus far only be applied for  50 % under almost equal forcing. Grounding-line positions differ by up to 49 km, show significant hysteresis, and migrate non-steadily in both scenarios with long quiescent phases disrupted by leaps of rapid migration. The simulations quantify the evolution of two different ice sheet geometries (namely thick and slow vs. thin and fast), triggered by the variable grounding-line migration over the differing ocean beds. Our study extends the timescales of 3D full-Stokes by an order of magnitude compared to previous studies with the help of parallelisation. The extended time frame for full-Stokes models is a first step towards better understanding other processes such as erosion and sediment redistribution in the ice shelf cavity impacting the entire catchment geometry

Continuous monitoring of the temporal evolution of the snowpack using upward-looking ground penetrating radar technology

Snow stratigraphy and water percolation are key parameters in avalanche forecasting. It is, however, difficult to model or measure stratigraphy and water flow in a sloping snowpack. Numerical modeling results depend highly on the type and availability of input data and the parameterization of the physical processes. Furthermore, the sensors themselves may influence the snowpack or be destroyed due to snow gliding and avalanches. Radar technology allows non-destructive scanning of the snowpack and deducing internal snow properties. If the radar system is buried in the ground, it cannot be destroyed by avalanche impacts or snow creep. During the winter seasons 2010-2011 and 2011-2012 we recorded continuous data with upward-looking pulsed radar systems (upGPR) at two test sites. We demonstrate that it is possible to determine the snow height with an accuracy comparable to conventional snow depth measuring devices. We determined the bulk volumetric liquid water content and tracked the position of the first stable wetting front. Wet-snow avalanche activity increased, when melt water penetrated deeper into the snowpack

Pre-site survey for deep drilling and new observations below Ekström Ice Shelf (Sub-EIS-Obs), Dronning Maud Land

During the last season and ongoing planning pre-site surveys are operated at the Ekströmisen, Dronning Maud Land, close to the Neumayer-Station III, with the primary target to build a stratigraphic age framework of the under-shelf-ice-sediments. These sediments are overlying the Explora Wedge, a syn- or post-rift volcanic deposit, and dipping north- to north-eastward. Expected ages could range from Late Mesozoic to Quaternary. From new vibroseismic profiles we will select sites for short core seafloor sampling of the oldest and of the youngest sediment sequences to confine their age time span. After that we could select one or several sites for potential deep drillings (several hundred-meter-deep) with the support of international partner if we could rise interest. The deep drillings should recover the sediments overlying the Explora Escarpment and should as well discover the nature of the Explora Wedge as well. We expect that the overlying sediment sequences could reveal the history of polar amplification and climate changes in this part of Antarctica, the build-up of the East Antarctic Ice Sheet during past warmer climates and its Cenozoic and future variability. The plan for seasons 2017/18 and 2018/19 are the testing of different sea floor sampling techniques through Hot Water Drill (HWD) holes. To select the drill sites for this shallow coring additional high resolution seismic will be acquired as well. Having holes through the shelf ice and sampling the sea floor will provide the unique opportunity for further piggy bag experiments consisting of multi-disciplinary nature. Experiments and measuring setup for oceanography, sea and shelf ice physics, geophysics, geology, hydrography, biogeochemistry could be planned to characterize the sea-ice and shelf ice system, underlying water column, and the sediments. Video characterization underneath the shelf ice and at the seafloor, sediment trap deployment, seafloor mapping with an AUV (Leng, DFKI, ROBEX) could lead as well to new ecosystem observations. Keywords: Deep geological drilling, EAIS build up and variability, Deglaciatio

In-situ observation of bubble trapping in polar firn

The air trapped in polar ice cores is not a direct record of past atmospheric composition but is strongly influenced by the process of firnification as bubbles are only sealed at a certain point, when the respective horizontal layer reaches a so called “critical” porosity. In order to investigate this process, we performed high-resolution (approximately 25μm) 3D-XCT measurements of the complete lock-in zone for two polar ice cores representing opposite extremes of the temperature and accumulation rate range: B53, close to Dome Fuji, East Antarctica and RECAP_S2, Renland, Greenland. For every 1m core segment, we scanned a minimum number of five sections of approximately 3.5cm height of the full core diameter with a focus on homogenous layers. This allows us to non-destructively deduce detailed profiles of open and closed porosity on a solid statistical basis. For each of the cores individually, we find that the trapping of bubbles in a single layer is solely determined by its total porosity and thereby independent of depth. We can confirm the existence of a distinct Schwander-type relation of closed and total porosity. Even though the two cores deviate from each other significantly in critical porosity, 0.0907 for B53 compared to 0.1025 for RECAP_S2, we observe many similarities. We hypothesize, that the determining factors of bubbletrapping are the average size and variability of pore space structures. This could potentially allow the reconstruction of past close-off porosities from the remaining pore structures in deep ice, e.g. from bubble number densities